Loading...
Surface-bound collagen 4 is significantly more stable than collagen 1
Stynes, Gil D. Kiroff, George K. Page, Richard S. Morrison, Wayne A. Kirkland, Mark A.
Stynes, Gil D.
Kiroff, George K.
Page, Richard S.
Morrison, Wayne A.
Kirkland, Mark A.
Abstract
Collagen 1 (C1) is commonly used to improve biological responses to implant surfaces. Here, the stability of C1 was compared with collagen 4 (C4) on a mixed macrodiol polyurethane, both adsorbed and covalently bound via acetaldehyde glow discharge polymerization and reductive amination. Substrate specimens were incubated in solutions of C1 and C4. The strength of conjugation was tested by incubation in 8 M urea followed by enzyme linked immunosorbent assays to measure residual C1 and C4. The basal lamina protein, laminin-332 (L332) was superimposed via adsorption on C4-treated specimens. Keratinocytes were grown on untreated, C1-treated, C4-treated, and C4 + L332-treated specimens, followed by measurement of cell area, proliferation, and focal adhesion density. Adsorbed C4 was shown to be significantly more stable than C1 and covalent conjugation conferred even greater stability, with no degradation of C4 over twenty days in 8 M urea. Cell growth was similar for C1 and C4, with no additional benefit conferred by superimposition of L332. The greater resistance of C4 to degradation may be consequent to cysteine residues and disulphide bonds in its non-collagenous domains. The use of C4 on implants, rather than C1, may improve their long-term stability in tissues.
Keywords
surface analysis, collagen, cell adhesion, interfaces, immobilization
Date
2017
Type
Journal article
Journal
Journal of Biomedical Materials Research Part A
Book
Volume
105
Issue
5
Page Range
1364-1373
Article Number
ACU Department
School of Behavioural and Health Sciences
Faculty of Health Sciences
Faculty of Health Sciences
Relation URI
Source URL
Event URL
Open Access Status
License
All rights reserved
File Access
Controlled